Archive for September, 2016

Next Steps

By • Sep 30th, 2016 • Category: Honors Thesis, Research

Now that the HPLC experiment is winding down and the outcome of the “cardenolide chase” is becoming clear – with exciting results – I’m getting ready to move on to a couple of new experiments. Those that follow will supplement the data generated already and will hopefully strengthen the overarching narrative of the project. During…
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Toxin Troubles

By • Sep 30th, 2016 • Category: Honors Thesis, Research

This month, I transformed several non-biofilm-forming (smooth) yeast strains with a high copy number plasmid containing a K28 killer toxin cDNA cassette and an antibiotic resistance marker. My transformations yielded a good number of resistant colonies, suggesting that I may have successfully generated strains with my toxin cassette. Unfortunately, I quickly ran into difficulty inducing…
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September Electrophysiology Experiments

By • Sep 30th, 2016 • Category: Honors Thesis, Research

I spent this month adjusting my patch clamp recording strategy to our lab setup in the lovely new ISC-3. There is a lot of equipment and finely tuned instruments that go into a complete electrophysiology recording assembly, so I was met with a lot of bugs in the system after it had been pretty disturbed…
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Humans are animals, but when are animals human?

By • Sep 30th, 2016 • Category: Honors Thesis, Research

Although it’s wonderful to be back in Williamsburg, I miss Boulder quite a lot. I’ve stopped watching films and am focusing on writing the outline and body of my thesis now, and honestly I’m experiencing major writer’s block (any tips on how to overcome this would be much appreciated). But I’m hoping that writing this…
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Transitioning into the school year

By • Sep 30th, 2016 • Category: Honors Thesis, Research

I can’t believe that it has been more than a month since school began! Despite the busyness of classes and other activities, the project has been steadily moving along. Phase 1 (creating the mutant strains) is finally beginning to wrap up (hopefully this doesn’t jinx it!), as my new J75 primers worked like a charm…
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September Update

By • Sep 30th, 2016 • Category: Honors Thesis, Research

Hi All! This month was all about preparation. The Young Lab recently welcomed a new graduate student into our lab, so I have been teaching him how to express protein from start to finish so that we can start work on a new (and very exciting) project involving the Glaser-Hay reaction. Unfortunately, the first time…
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Investigating Ancient Novels (Who Knew Those Were a Thing?)

By • Sep 30th, 2016 • Category: Honors Thesis, Research

Over the summer, I dove into a whole bunch of texts from the Second Sophistic (that is, Greek literature from the first to third centuries A.D., AKA “Imperial Greek Literature” since it happens at the same time that the Roman emperors rule the Mediterranean).  Anyway, one genre among many (oratory, philosophy, satire, etc.) from that…
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September Update

By • Sep 30th, 2016 • Category: Honors Thesis, Research

September has been a difficult month for the C9 project, but we have gotten the majority of the necessary data in for our nucleophagy paper, and are hopefully going to shift towards a more cancer-cell based approach for the new project. Virus/plasmid construction has been problematic, and although we plan on continuing to work on…
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Books Books Books

By • Sep 30th, 2016 • Category: Honors Thesis, Research

Poems, laws, memories, statistics, dissertations… I’m in the thick of it. Every week, books trickle in on interlibrary loan; every day, new articles show themselves on Swem’s many databases. I am still in the collecting phase, figuring out what is relevant and where I am in Guatemala’s complex history. Earlier this week, I read an…
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Why I Should Never Trust Myself

By • Sep 29th, 2016 • Category: Honors Thesis, Research

It seems like over this past month, I’ve fallen even more in love with my project. A good part of that is likely because I recently was looking back over my NMRs and made a wonderful discovery. Over the summer, I was attempting to synthesize a compound called Rhodamin 6G-diethoxyaniline (R6G-dEA). R6G is a tricky…
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