Author Archive

Defense and Future Plans for the Trojan Horse Project

By • Apr 28th, 2017 • Category: Honors Thesis, Research

On Tuesday, I successfully defended my Honors thesis! I am very excited to conclude this long journey. I now just have to make some small edits to my written thesis before submitting it to the archives. I plan to stay in contact with my thesis advisor as other students continue the Trojan Horse project. Future…
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Final Stretch!

By • Mar 30th, 2017 • Category: Honors Thesis, Research

This month, I succeeded in transforming three strains to express the K2 killer toxin on an extrachromosomal plasmid. I am now conducting one last experiment for the semester, competing these engineered strains with a biofilm-forming strain under different initial strain proportions. I will be photographing and processing colonies over the next few days. I am…
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Results and Presentations

By • Feb 26th, 2017 • Category: Honors Thesis, Research

This past month, I have focused on finishing data collection for the biofilm/toxin competition assay I conducted last month. After processing the rest of my stored colonies, I calculated initial and final relative frequencies of the biofilm-forming strains in the assay and visualized these data graphically. The resulting graphs illustrate clearly that toxin production affects…
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Continuing Biofilm/Toxin Assays: Progress!

By • Jan 31st, 2017 • Category: Honors Thesis, Research

I have been putting a lot of time into lab so far this semester. A lot of my work has been towards generation of my Trojan Horse strain. I have done transformations with both an extrachromosomal plasmid and with a cassette targeted for integration into the yeast genome. I am now assaying potentially successful transformants…
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The post Continuing Biofilm/Toxin Assays: Progress! appeared first on W&M Honors Fellows.



Post-Winter-Break Plans

By • Dec 30th, 2016 • Category: Honors Thesis, Research

What with finals and break, I have had to put my work in the lab on hold for a few weeks; however, this time has given me the opportunity to think about my plans for the future and specifically about what I hope to accomplish next semester. As mentioned in my last post, our lab…
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November Progress

By • Nov 30th, 2016 • Category: Honors Thesis, Research

This month, I repeated my assay of toxin and biofilm interactions on four different types of media, using both K2 and K28 toxin-producing strains and two different biofilm-forming strains. I successfully took light and fluorescent images of all the pure and mixed colonies from my assay, and am now processing the colonies in order to…
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Moving Forward

By • Oct 31st, 2016 • Category: Honors Thesis, Research

After my last post, I finally succeeded in visualizing K28 toxin production after testing several different potentially sensitive strains. I then used these strains to test for toxin production by the strains I had previously transformed with my K28 plasmid. Sadly, the transformants did not show toxin activity. I am now changing my approach in…
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Toxin Troubles

By • Sep 30th, 2016 • Category: Honors Thesis, Research

This month, I transformed several non-biofilm-forming (smooth) yeast strains with a high copy number plasmid containing a K28 killer toxin cDNA cassette and an antibiotic resistance marker. My transformations yielded a good number of resistant colonies, suggesting that I may have successfully generated strains with my toxin cassette. Unfortunately, I quickly ran into difficulty inducing…
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New Plasmid!

By • Aug 31st, 2016 • Category: Honors Thesis, Research

After a few weeks at home, I am now back on campus and returning to my research. After struggling to transform yeast strains with a cassette that would be incorporated into the genome, I am now instead going to use an extrachromosomal plasmid. After a few days of preparation, I am ready to attempt transformation…
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New Plasmid!

By • Aug 31st, 2016 • Category: Honors Thesis, Research

After a few weeks at home, I am now back on campus and returning to my research. After struggling to transform yeast strains with a cassette that would be incorporated into the genome, I am now instead going to use an extrachromosomal plasmid. After a few days of preparation, I am ready to attempt transformation…
Read More