Author Archive

Special Thanks and What the Future Holds

By • Apr 28th, 2016 • Category: Honors Thesis, Research

Acknowledgments: I would just like to take the time to thank all of the people who have made my research experience here at William & Mary possible. First and foremost, my principal investigator Dr. Mark Forsyth has been the best role model, supporter, encourager, and friend a student could ever ask for. Mark has been…
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Conclusions

By • Apr 28th, 2016 • Category: Honors Thesis, Research

“Previous studies have connected the importance of H. pylori adherence to induction of host immune response (Ishijimi et al., 2011; Oleasetro et al., 2008). In particular, SabA is a key protein of interest. SabA-positive status is positively correlated with the formation of gastric pathologies in Western H. pylori strains (Yamaoka et al., 2002). In 2005,…
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Future Directions

By • Apr 28th, 2016 • Category: Honors Thesis, Research

Here are some ideas for future experiments as an extension of my work with sabA: Examine whether SabA-mediated increased adherence increases CagA translocation and NF-KB activation in AGS cells Examine function of SabB. Is it an adhesin? If not, what is it’s function? SabA-mediated adhesion may not increase IL-8, but how about HopD-mediated adhesion? HopZ? OipA?…
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You cannot have success without first having failure

By • Apr 28th, 2016 • Category: Honors Thesis, Research

sabA has a homolog gene sabB which originated from a duplication event sometime in H. pylori‘s genetic history. Because of high sequence similarity between the two genes, we hypothesized that SabB may mimic SabA’s function as an adhesin protein (the function of SabB is not yet known). To test this, we wanted to turn the sabB gene phase…
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Final Results 4!

By • Apr 28th, 2016 • Category: Honors Thesis, Research

In a previous study done by a former lab member, they found that sabA was regulated by the protein ArsS, which is part of a two-component signal transduction system that senses the environment and alters gene regulation appropriately. ArsRS responds to environmental acidity. The more acidic the environment, the greater the TCST activation. Because sabA is a…
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Final Results Part 3!

By • Apr 28th, 2016 • Category: Honors Thesis, Research

H. pylori comes in two flavors: cag positive and cag negative. cag positive strains are linked with the formation of H. pylori dependent gastric pathologies such as gastritis, ulcer formation, and gastric adenocarcinoma. These strains contain a pathogenicity island of DNA encoding for a type IV secretion system (T4SS) which injects a toxic effector protein called CagA into gastric epithelial…
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Final Results Part 2!

By • Apr 28th, 2016 • Category: Honors Thesis, Research

In the last post, I talked about how poly-T tract mutations and phase on mutations are not additive on sabA transcription frequency. Interestingly, adhesion studies back this up. We have a cancerous gastric epithelial cell line (AGS cells) that we infected with phase on and phase off H. pylori each with either 18 (WT) thymines or 23…
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Final Results Part 1!

By • Apr 28th, 2016 • Category: Honors Thesis, Research

Hello everyone! It’s been a while since I’ve posted so I’m going to update all of you on the interesting results we found! We found that turning the sabA gene phase on and altering the poly-T tract length to extend it by five thymines did not have additive effects on transcription. We think this may be due…
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One Step at a Time

By • Oct 30th, 2015 • Category: Honors Thesis, Research

It’s been a while since I’ve posted, but I have been hard at work! There has also been some slow-coming, but exciting progress thus far. As a recap, here are my research questions once more and progress for each research question: 1) In hopZ gene expression regulated by slip-strand mispairing of the poly-A tract? We…
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Good to be back!

By • Aug 22nd, 2015 • Category: Honors Thesis, Research, undergraduate research

I arrived back on campus a week ago, and I jumped straight into my research again! I have a lot of projects going on, so bear with me. HopZ adhesin expression and regulation: As a recap, I am looking at mutations in the poly-adenine (poly-A) tract and poly-CT tract and how they affect hopZ expression. Mutating the…
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